Draft genome sequence of a multidrug-resistant Pseudomonas aeruginosa producing blaSIM metallo-β-lactamase: London, UK

Abstract

ABSTRACT: Objectives: We report the draft genome sequence of a multidrug-resistant Pseudomonas aeruginosa isolate (PA3) producing SIM carbapenemase from a patient in the United Kingdom. Methods: Isolation, identification and antimicrobial susceptibility testing were performed according to local routine microbiology protocols. Whole genomic DNA was sequenced using an Illumina HiSeq platform. The generated reads were de novo assembled using SPAdes version 3.7.1. Annotation was performed by the NCBI Prokaryote Genome Annotation Pipeline version 4.6. Sequence type, antimicrobial resistance and virulence-related genes were predicted from the sequence. Results: P. aeruginosa PA3 was resistant to most antipseudomonal β-lactams, aminoglycosides and quinolones but susceptible to colistin. The assembly comprised 100 contigs (>1000 bp) with a total length of 7 485 621 bp, and a total of 7190 coding sequences. The isolate belonged to sequence type (ST) 446, serotype O11 and contained blaSIM-1 metallo-β-lactamase in a class 1 integron structure. Conclusion: We report a blaSIM-1 containing multidrug-resistant strain of P. aeruginosa from the UK. Moreover, the isolate was ST 446, a genetic background with potential for greater global dissemination.

Keywords

• Carbapenems
• P. aeruginosa
• SIM metallo-β-lactamase