Investigation of the Possible Role of Macrophage Migration Inhibitory Factor Gene -173G/C Polymorphism ın Patients with Atherosclerosis

Abstract

Purpose: Atherosclerosis is defined as an inflammatory disease that results in the formation of atherosclerotic plaques caused by the deposition of cholesterol in the arterial intima. As a result of damage to the intima layer of the vessel, foam cell formation following cholesterol accumulation and plaque development due to smooth muscle cell increase are observed. In different stages of atherosclerosis, migration of leukocytes to the vessel wall is provided by functional type chemokines; ıt was aimed to investigate the possible role of MIF -173G/C polymorphism in atherosclerosis disease depending on this function, since it has the same functional properties as chemokines. Materials and Methods: Thirty patients with 70% occlusion detected by angiography and 30 healthy individuals were included in the study. DNA isolation was performed with DNA isolation kit from blood samples taken into EDTA tubes from individuals participating in the study. Analysis of MIF -173G/C polymorphism was performed on Real Time PCR (LC480, Roche). Statistical analyzes were performed with the program STATISTICA version 13.5.0.17 (TIBCO Software Inc. (2017)). Statistical significance level p≤ 0.05 was taken in all comparisons. Results: The frequency of GG, GC and CC genotypes in the MIF -173G/C polymorphism was 55.26%, 41.18% and 66.66% in the patient group, respectively, and 44.74%, 58.82% and 33.33% in the control group. When compared with the GG genotype, it was determined that those with the GC genotype had a 0.567-fold (p=0.3367) risk of developing the disease and those with the CC genotype had a 1.6190-fold (p= 0.7038) risk of developing the disease. Conclusion: It was determined that those with the C allele in the MIF-173 G/C polymorphism pose a risk for atherosclerosis disease.

Keywords

• mif
• pcr
• aterosclerosis