Laboratory Animal Research (Dec 2016)

Diallyl disulfide attenuates acetaminophen-induced renal injury in rats

  • Jin-Young Shin,
  • Ji-Hee Han,
  • Je-Won Ko,
  • Sung-Hyeuk Park,
  • Na-Rae Shin,
  • Tae-Yang Jung,
  • Hyun-A Kim,
  • Sung-Hwan Kim,
  • In-Sik Shin,
  • Jong-Choon Kim

DOI
https://doi.org/10.5625/lar.2016.32.4.200
Journal volume & issue
Vol. 32, no. 4
pp. 200 – 207

Abstract

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Abstract This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NCAL), which are novel biomarkers of nephrotoxicity in renal tissues, in response to AAP treatment. The following four experimental groups were evaluated: (1) vehicle control, (2) AAP (1,000 mg/kg), (3) AAP&DADS, and (4) DADS (50 mg/kg/day). AAP treatment caused acute kidney injury evidenced by increased serum blood urea nitrogen (BUN) levels and histopathological alterations. Additionally, Western blot and immunohistochemistry analysis showed increased expression of KIM-1 and NCAL proteins in renal tissues of AAP-treated rats. In contrast, DADS pretreatment significantly attenuated the AAP-induced nephrotoxic effects, including serum BUN level and expression of KIM-1 and NCAL proteins. Histopathological studies confirmed the renoprotective effect of DADS. The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NCAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure.

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