Nature Communications (Jun 2016)

Long-read sequencing and de novo assembly of a Chinese genome

  • Lingling Shi,
  • Yunfei Guo,
  • Chengliang Dong,
  • John Huddleston,
  • Hui Yang,
  • Xiaolu Han,
  • Aisi Fu,
  • Quan Li,
  • Na Li,
  • Siyi Gong,
  • Katherine E. Lintner,
  • Qiong Ding,
  • Zou Wang,
  • Jiang Hu,
  • Depeng Wang,
  • Feng Wang,
  • Lin Wang,
  • Gholson J. Lyon,
  • Yongtao Guan,
  • Yufeng Shen,
  • Oleg V. Evgrafov,
  • James A. Knowles,
  • Francoise Thibaud-Nissen,
  • Valerie Schneider,
  • Chack-Yung Yu,
  • Libing Zhou,
  • Evan E. Eichler,
  • Kwok-Fai So,
  • Kai Wang

DOI
https://doi.org/10.1038/ncomms12065
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 10

Abstract

Read online

Short-read sequencing has inherent limitations in the characterisation of long repeat elements. Shi and Guo et al.combine single-molecule real-time sequencing and IrysChip to construct a Chinese reference genome that fills many gaps in the reference genome, and identify novel spliced genes.