Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy

Jana Verbančič; Jenny Jiahui Huang; Heather E. McFarlane

STAR Protocols (Dec 2021)

Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy

Jana Verbančič,
Jenny Jiahui Huang,
Heather E. McFarlane

Affiliations

Jana Verbančič: School of Biosciences, University of Melbourne, Parkville, VIC 3010, Australia; Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Potsdam, Germany; Corresponding author
Jenny Jiahui Huang: Department of Cell and Systems Biology, University of Toronto, 25 Harbord St., Toronto, ON M5S 3G5, Canada
Heather E. McFarlane: Department of Cell and Systems Biology, University of Toronto, 25 Harbord St., Toronto, ON M5S 3G5, Canada; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100863

Abstract

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Summary: We describe sample preparation and visualization of fluorescently tagged cellulose synthases in cellulose synthase complexes at the plasma membrane of Arabidopsis hypocotyl epidermal cells using live-cell imaging via spinning disk microscopy. We present a technique for sample mounting that may be suitable for imaging other samples. Additionally, we offer free, open-source solutions for image analysis and provide extensive troubleshooting suggestions.For complete information on the use and execution of this protocol, please refer to McFarlane et al., 2021.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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