Nature Communications (Jan 2017)

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus

  • Anne Bothmer,
  • Tanushree Phadke,
  • Luis A. Barrera,
  • Carrie M Margulies,
  • Christina S. Lee,
  • Frank Buquicchio,
  • Sean Moss,
  • Hayat S. Abdulkerim,
  • William Selleck,
  • Hariharan Jayaram,
  • Vic E. Myer,
  • Cecilia Cotta-Ramusino

DOI
https://doi.org/10.1038/ncomms13905
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 12

Abstract

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CRISPR-Cas9 has rapidly become a common molecular biology tool for modifying genomes and has been modified to generate single-strand nicks as well as double-strand breaks. Here the authors explore the DNA repair pathways activated by the different variants of Cas9.