Journal of the Selva Andina Research Society (Aug 2013)
Molecular markers validation to drought resistance in wheat meal (Triticum aestivum L.) under greenhouse conditions
Abstract
With the aim to study the genetic resistance to drought and validate molecular markers co-localized with genes/QTLs for this factor, 16 varieties were evaluated as well as advanced lines of wheat meal (Triticum aestivum) in two stages of crop development. Physiological parameters were considered: amount of chlorophyll (clo), wilting or severity degree (SEV) and recovery (reco), morphological parameters: foliage dry matter (FDM) and root dry matter (RDM), the integrated resistance mechanisms: water use efficiency (WUE), other parameters: number of grains (Ngrain) and grain weight (Wgrain), biochemical parameters: Catalaza (CAT), Ascorbate Peroxidase (APX) and Guaiacol Peroxidase (POX) and three microsatellite markers (Xwmc603, Xwmc596, Xwmc9). Results showed significant differences for MSR and Ngrain. It was observed that Anzaldo, ERR2V.L-20, EARII2V.L-5, EARIZV.L-11, ERR2V.L-11 and EE2V.L-19 were the most resistant to drough water stress. There was a highly significant negative correlation between the MSR and Ngrain. All other variables showed low and non-significant correlations. In biochemical analyzes, the Anzaldo variety showed an increased enzymatic activity compared to controls in all cases (CAT-APX and POX), being the most resistant to water stress by drought. Finally, it was found that SSR markers (Xwmc596 and Xwmc9) are co-located with the gene / QTL of drought resistance and can be used for marker-assisted selection.
