Life (Jul 2023)

In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study

  • Bolat Seisenov,
  • Dulat Duimbayev,
  • Nurlybay Kazhgaliyev,
  • Talgat Abdrakhmanov,
  • Alexandra Tegza,
  • Rustem Abeldinov,
  • Nadezhda Burambayeva,
  • Alma Temirzhanova,
  • Ivan Tegza,
  • Zhomart Kemeshev,
  • Assylbek Zhanabayev,
  • Nurbolat Akhmetbekov,
  • Marat Aisin,
  • Kuandyk Zhugunissov,
  • Arman Issimov

DOI
https://doi.org/10.3390/life13081632
Journal volume & issue
Vol. 13, no. 8
p. 1632

Abstract

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In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (p ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms.

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