Application of Dot-Immunoassay for Detection of Plague Agent Antigens in the Field Samples

Abstract

CFU/ml) and soluble antigens (FI) − ≥ 4.8 ng/ml and high specificity, confirmed by the absence of false-positive reactions with five heterologous microorganisms. The test-systems were used for Y. pestis antigen detection in field material from the territory of the Altai mountain natural plague focus by dot-immunoassay with comparison of the received results in passive hemagglutination reaction. Test-systems possessed a number of advantages as compared to routine serological reactions and could be applied with success by practical public health services both in stationary and field conditions.

Keywords

• plague agent
• specific antibodies
• colloid silver
• dot-immunoassay