Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex

Junqiao Jia; Tarek Hilal; Katherine E. Bohnsack; Aleksandar Chernev; Ning Tsao; Juliane Bethmann; Aruna Arumugam; Lane Parmely; Nicole Holton; Bernhard Loll; Nima Mosammaparast; Markus T. Bohnsack; Henning Urlaub; Markus C. Wahl

doi:10.1038/s41467-023-37528-3

Nature Communications (Apr 2023)

Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex

Junqiao Jia,
Tarek Hilal,
Katherine E. Bohnsack,
Aleksandar Chernev,
Ning Tsao,
Juliane Bethmann,
Aruna Arumugam,
Lane Parmely,
Nicole Holton,
Bernhard Loll,
Nima Mosammaparast,
Markus T. Bohnsack,
Henning Urlaub,
Markus C. Wahl

Affiliations

Junqiao Jia: Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Structural Biochemistry
Tarek Hilal: Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Structural Biochemistry
Katherine E. Bohnsack: Universitätsmedizin Göttingen, Department of Molecular Biology
Aleksandar Chernev: Max-Planck-Institut für Multidisziplinäre Naturwissenschaften, Bioanalytical Mass Spectrometry
Ning Tsao: Washington University School of Medicine, Department of Pathology & Immunology and Center for Genome Integrity
Juliane Bethmann: Max-Planck-Institut für Multidisziplinäre Naturwissenschaften, Bioanalytical Mass Spectrometry
Aruna Arumugam: Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Structural Biochemistry
Lane Parmely: Washington University School of Medicine, Department of Pathology & Immunology and Center for Genome Integrity
Nicole Holton: Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Structural Biochemistry
Bernhard Loll: Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Structural Biochemistry
Nima Mosammaparast: Washington University School of Medicine, Department of Pathology & Immunology and Center for Genome Integrity
Markus T. Bohnsack: Universitätsmedizin Göttingen, Department of Molecular Biology
Henning Urlaub: Max-Planck-Institut für Multidisziplinäre Naturwissenschaften, Bioanalytical Mass Spectrometry
Markus C. Wahl: Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of Structural Biochemistry

DOI: https://doi.org/10.1038/s41467-023-37528-3
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 14

Abstract

Read online

ASCC3 is a multi-functional helicase that contains two consecutive Ski2-like helicase units. Here, the authors show that ASCC3 can unwind DNA by threading one strand of a substrate duplex through both helicase units, supported by the TRIP4 protein.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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