Nature Communications (Aug 2020)
Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis
- Lu Han,
- Praneet Chaturvedi,
- Keishi Kishimoto,
- Hiroyuki Koike,
- Talia Nasr,
- Kentaro Iwasawa,
- Kirsten Giesbrecht,
- Phillip C. Witcher,
- Alexandra Eicher,
- Lauren Haines,
- Yarim Lee,
- John M. Shannon,
- Mitsuru Morimoto,
- James M. Wells,
- Takanori Takebe,
- Aaron M. Zorn
Affiliations
- Lu Han
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Praneet Chaturvedi
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Keishi Kishimoto
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Hiroyuki Koike
- CuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Talia Nasr
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Kentaro Iwasawa
- CuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Kirsten Giesbrecht
- CuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Phillip C. Witcher
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Alexandra Eicher
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Lauren Haines
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Yarim Lee
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- John M. Shannon
- Division of Pulmonary Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Mitsuru Morimoto
- Laboratory for Lung Development, RIKEN Center for Biosystems Dynamics Research (BDR)
- James M. Wells
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Takanori Takebe
- CuSTOM, Division of Gastroenterology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- Aaron M. Zorn
- Center for Stem Cell and Organoid Medicine (CuSTOM), Perinatal Institute, Division of Developmental Biology, Cincinnati Children’s Hospital, Department of Pediatrics, University of Cincinnati, College of Medicine
- DOI
- https://doi.org/10.1038/s41467-020-17968-x
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 16
Abstract
The fetal murine foregut develops into visceral organs via interactions between the mesoderm and endoderm, but how is unclear. Here, the authors use single cell RNAseq to show a diversity in organ specific splanchnic mesoderm cell-types, infer a signalling network governing organogenesis and use this to differentiate human pluripotent stem cells.
