Determination of 3 ingredients in Anshenbao granules by HPLC-ELSD

Abstract

In order to improve the quality control standard of Anshenbao granules, a high performance liquid chromatography (HPLC) method is developed for the determination of three active ingredients including Jujuboside A, Scopoletin and Quercetin in Anshenbao granules. The analysis is performed on a Agilent Zorbox SB C18 column(4.6 mm×150 mm,5 μm) with a gradient mobile phase of methanol-0.5% glacial acetic acid at a flow rate of 1.0 mL/min. The evaporative light scattering detector(ELSD) is used, the column temperature is 35 ℃, and the injection is 10 μL. The three active ingredients are separated completely under the given chromatogram system with a good linearity (r=0.995 6~0.998 0) of peak area and the injection. The results of precision, repeatability and stability experiments can meet the requirement, and the mean recoveries of each active ingredients are all in the range of 94.74%～96.08%. The analytical method is simple, accurate and sensitive, and it can be used for determination of active ingredients in Anshenbao granules. It is of practical significance to improve the quality standard of Anshenbao and improve the quality control of medicine.

Keywords

• chromatographic analysis
• HPLC
• Anshenbao granules
• jujuboside A
• scopoletin
• quercetin
• quality standard