Effect of Jiangteng Tea on the Cold Coagulation Stasis Dysmenorrhea in Rats

Abstract

Jiangteng tea is composed of brown sugar,ginger,Teng tea,xylooligosaccharide and chrysanthemum.To observe the pharmacodynamic test of Jiangteng tea on cold blood stasis dysmenorrhea,and provide reference materials for the clinical trials,a dysmenorrhea model was prepared by injecting estradiol benzoate,and ginger vine tea was administered simultaneously by intragastric administration for 10 days.One hour after the last administration,10 U of oxytocin was injected intraperitoneally.And the rat's pain response was observed within 30 minutes.The writhing response was selected as an indicator of strong uterine contraction (dysmenorrhea),the concentration of β-endorphin (β-EP) and 6-ketoprostaglandin F1α were measured,at the same time,the uterus and ovaries were taken and HE stained for histological examination.The results showed that Jiangteng tea can reduce the number of writhing in rats with dysmenorrhea,increase the levels of β-endophorin and 6-ketoprostaglandin F1α,and have a better effect on improving dysmenorrhea.At the same time,an ice bath + epinephrine hydrochloride was used to prepare a cold coagulation blood stasis model,and Jiangteng tea was administered by gavage simultaneously for 7 days with hemorheological indexes and hematocrit were measured.Compared with the model group,the hematocrit in the drug group was reduced,but there was no significant difference (P＞0.05).The low-shear viscosity and low-shear relative value of whole blood in the Jiangtengcha medium-dose group were significantly reduced (P＜0.05).In the high-dose Jiangteng tea group,whole blood low,medium and high shear viscosity,low shear relative value and erythrocyte aggregation index were significantly reduced(P＜0.01),and Kasson viscosity was significantly reduced (P＜0.05).The results showed that Jiangteng tea can reduce the whole blood viscosity and plasma viscosity of rats with cold coagulation and blood stasis,suggesting that Jiangteng tea has certain improvement on cold coagulation and blood stasis syndrome.These studies showed that Jiangteng tea has a certain effect on dysmenorrhea and cold blood stasis,and has the functions of soothing dysmenorrhea.

Keywords

• jiangteng tea
• dysmenorrhea
• cold coagulation stasis
• β-endoceptide
• 6 ketoprostaglandin f1α