RESEARCH OF MICROBIOLOGICAL SYNTHESIS OF BIOLOGICALLY ACTIVE SUBSTANCES IN SOLUTIONS OF BEET MOLASSES

Abstract

Treatment of solutions of beet molasses with 5 strains of microorganisms belonging to 4 species in order to enrich the solutions with β-carotene, vitamin B2 and organic acids have been studied. The influence of the initial pH value, as well as the optimal dry matter content in molasses solutions, which ensure the effective vital activity of microorganisms with the synthesis of biologically active substances, has been revealed. It has been found that F-3758 and F-3759 Blakeslea trispora strains in molasses solutions are not capable of synthesizing β-carotene. It has been found that B-501 Bacillus subtilis strain exhibits high activity in molasses solutions with initial pH values of 7.0 and 8.5 and a dry matter content of not more than 20 %. As a result of life activity in molasses solutions of Bacillus subtilis, strain B-501 synthesizes vitamin B2 in the amount of 1.42-1.93 mg/g of sugars. It has been revealed that Y-2482 Debaryomyces hansenii strain in molasses solution with an initial pH of 7.0 synthesizes organic acids in an amount of 1.790 (mmol/100 ml)/g of sugars. In addition, Y-2482 Debaryomyces hansenii strain is capable of vital activity in solution with an initial pH of 8.5, but some of its vital products are neutralized. It has been established that Y-2305 Guehomyces pullulans is capable of vital activity in molasses solutions, however, its vital products are not only organic acids, but also some compounds with oxy groups, as a result of which they are neutralized. Ultimately, the amount of synthesized Y-2305 Guehomyces pullulans organic acids is 0.2 (mmol/100 ml)/g of sugar. It has been proved that B-501 Bacillus subtilis strain and Y-2482 Debaryomyces hansenii strain are promising strains for enriching beet molasses solutions with biologically active substances.

Keywords

• molasses
• synthesis
• vitamins
• organic acids
• microorganisms
• vital functions