3-hydroxyflavone-bovine serum albumin interaction in Dextran medium

Abstract

Spectroscopic analysis of a bioactive flavonol, 3-Hydroxyflavone (3-HF), in systems based on Dextran 70 (Dx70) (an important bio-relevant polysacharide) and Bovine Serum Albumin (BSA) (a carrier protein), have been studied by fluorescence and circular dichroism. Changes produced by different concentrations of Dx70 on the fluorescent characteristics of 3-HF, and on the excited - state intramolecular proton transfer (ESIPT) process were studied. The influence of 3-HF binding and of Dx70 on the secondary structure of BSA were investigated by circular dichroism spectroscopy. The influence of temperature (30-80°C range) on the intrinsic Tryptophan fluorescence in 3-HF/BSA/Dx70 systems, was investigated. The results are discussed with relevance to 3-HF as a sensitive fluorescence probe for exploring flavone-protein interaction in plasma expander media and also for its biological evaluation.

Keywords

• flavones
• proteins
• dextran 70
• fluorescence spectroscopy
• ESIPT