Diversity analysis of potato (Solanum spp.) with microsatellite molecular markers of Secclla and Santo Tomas de Pata (Huancavelica) and Santillana (Ayacucho) districts

Abstract

The research was carried out with the objectives of studying the molecular profiles of genetic diversity, comparing the variability and genetic distances of the molecular markers of potatoes (Solanum spp.) which were collected in farmers' field from 12 communities of the Secclla, Santo Tomás de Pata and Santillana districts. The 128 accessions were cultivated in polyethylene bags with two repetitions each. The sampling of the young leaflets was carried out 48 days after sowing. DNA extraction was performed by CTAB method, copies of DNA fragments were obtained by PCR, which were separated by PAGE. In the molecular analysis, 8 pairs of microsatellite markers were used. The results were analyzed by the statistics, Simple Matching coefficient, NTSYS 2.10p, UPGMA grouping method to estímate the polymorphism and the AMOVA was calculated using the Arlequín program (versión 3.0). The average PIC was 0.238, indicating the existence of polymorphism. The distance and genetic similarity, the populations that exhibit the greatest genetic relationship include Santillana and Santo Tomás de Pata, denoting that the accessions of these populations have greater common characteristics. The AMOVA shows that the variability is within populations (91.55%), and the genetic differentiation between the three populations is modérate (FST = 0.08448).

Keywords

• genetic diversity of solanum spp.
• microsatélite
• ayacucho-huancavelica
• potato polymorphism
• ssr markers.