Distinguishing isolates of cereal stem disease pathogens of the genus Tapesia from isolates of Fusarium and Rhizoctonia using the RAPD method

Abstract

The RAPD method (Random Amplification of Polymorphous DNA) was used to distinguish Tapesia acufonnis and T. yallundae isolates from other pathogens of wheat stem base diseases (Fusarium avenaceum, F. culmorum, F. nivale, Rhizoctonia cerealis and R. so/ani).Isolates of the fungi originated from infected wheat plants (Triticum aestivum L.) collected at various locations in the Czech Republic. Three decameric oligonucleotide-primers were selected as producing genotype-specific RAPD products. These markers distinguish all isolates of the above taxons. The intra-species genetic variability of these species also was studied. The values of Dice's coefficients of similarity suggest that isolates of Tapesia yallundae showed a higher degree of intra-species variability than those ofT acufonnis. A high degree of intra-species variability was identified also in Fusarium avenaceum.

Keywords

• dna
• polymerase chain reaction (pcr)
• random amplified polymorphic dna (rapd)
• markers
• fingerprinting
• genetic similarity