Heterozygous diploid and interspecies SCRaMbLEing

Michael J. Shen; Yi Wu; Kun Yang; Yunxiang Li; Hui Xu; Haoran Zhang; Bing-Zhi Li; Xia Li; Wen-Hai Xiao; Xiao Zhou; Leslie A. Mitchell; Joel S. Bader; Yingjin Yuan; Jef D. Boeke

doi:10.1038/s41467-018-04157-0

Nature Communications (May 2018)

Heterozygous diploid and interspecies SCRaMbLEing

Michael J. Shen,
Yi Wu,
Kun Yang,
Yunxiang Li,
Hui Xu,
Haoran Zhang,
Bing-Zhi Li,
Xia Li,
Wen-Hai Xiao,
Xiao Zhou,
Leslie A. Mitchell,
Joel S. Bader,
Yingjin Yuan,
Jef D. Boeke

Affiliations

Michael J. Shen: Department of Biochemistry Molecular Pharmacology and Institute for Systems Genetics, NYU Langone Health
Yi Wu: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Kun Yang: Department of Biomedical Engineering, Johns Hopkins University
Yunxiang Li: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Hui Xu: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Haoran Zhang: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Bing-Zhi Li: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Xia Li: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Wen-Hai Xiao: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Xiao Zhou: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Leslie A. Mitchell: Department of Biochemistry Molecular Pharmacology and Institute for Systems Genetics, NYU Langone Health
Joel S. Bader: Department of Biomedical Engineering, Johns Hopkins University
Yingjin Yuan: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University
Jef D. Boeke: Department of Biochemistry Molecular Pharmacology and Institute for Systems Genetics, NYU Langone Health

DOI: https://doi.org/10.1038/s41467-018-04157-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

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SCRaMbLE has been used to rearrange synthetic chromosomes that have been introduced into host yeast. Here the authors produce semi-synthetic heterozygous diploid strains for rapid selection of phenotypes and map the rearrangements underlying selected phenotypes such as thermoresistance and caffeine resistance.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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