A Novel Method for Sensitive Detection of <i>Vibrio alginolyticus</i> Based on Aptamer and Hybridization Chain Reaction in Aquaculture

Yifan Zhao; Sheng Luo; Zhaohui Qiao; Qianjin Zhou; Jianzhong Fan; Jianfei Lu; Jiong Chen

doi:10.3390/fishes8100477

Fishes (Sep 2023)

A Novel Method for Sensitive Detection of <i>Vibrio alginolyticus</i> Based on Aptamer and Hybridization Chain Reaction in Aquaculture

Yifan Zhao,
Sheng Luo,
Zhaohui Qiao,
Qianjin Zhou,
Jianzhong Fan,
Jianfei Lu,
Jiong Chen

Affiliations

Yifan Zhao: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China
Sheng Luo: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China
Zhaohui Qiao: College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China
Qianjin Zhou: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China
Jianzhong Fan: Ningbo Boao Biological Engineering Limited Company, Ningbo 315211, China
Jianfei Lu: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China
Jiong Chen: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China

DOI: https://doi.org/10.3390/fishes8100477
Journal volume & issue: Vol. 8, no. 10
p. 477

Abstract

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In this study, we developed a novel method for the detection of Vibrio alginolyticus, utilizing the specific recognition of an aptamer for V. alginolyticus and signal amplification via hybridization chain reaction (HCR) and horseradish-peroxidase-conjugated streptavidin. The proposed HCR-based multivalent aptamer (multi-Apt) amplifier allows for sensitive detection of V. alginolyticus in a linear range from 10 to 107 CFU/mL. The linear equation is y = 747.5x + 126.2, R2 = 0.986, and the limit of detection (LOD) is 3 CFU/mL. Seawater and freshwater samples were utilized in the spike recovery experiment, yieldng a recovery rates ranging from 94.3% to 108.8%. The relative standard deviation (RSD) for all samples is below 6.73%. Taken together, the proposed method has great potential for application in monitoring of V. alginolyticus in aquaculture environments.

Published in Fishes

ISSN: 2410-3888 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://www.mdpi.com/journal/fishes

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