mSphere (Aug 2021)

Mutagenesis Mapping of RNA Structures within the Foot-and-Mouth Disease Virus Genome Reveals Functional Elements Localized in the Polymerase (3D <sup>pol</sup> )-Encoding Region

  • Lidia Lasecka-Dykes,
  • Fiona Tulloch,
  • Peter Simmonds,
  • Garry A. Luke,
  • Paolo Ribeca,
  • Sarah Gold,
  • Nick J. Knowles,
  • Caroline F. Wright,
  • Jemma Wadsworth,
  • Mehreen Azhar,
  • Donald P. King,
  • Tobias J. Tuthill,
  • Terry Jackson,
  • Martin D. Ryan

DOI
https://doi.org/10.1128/mSphere.00015-21
Journal volume & issue
Vol. 6, no. 4

Abstract

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Some RNA structures formed by the genomes of RNA viruses are critical for viral replication. Our study shows that of 46 conserved RNA structures located within the regions of the foot-and-mouth disease virus (FMDV) genome that encode the nonstructural proteins, only 3 are essential for replication of an FMDV subgenomic replicon.