iScience (Mar 2020)
A Glial-Neuronal Circuit in the Median Eminence Regulates Thyrotropin-Releasing Hormone-Release via the Endocannabinoid System
- Erzsébet Farkas,
- Edina Varga,
- Balázs Kovács,
- Anett Szilvásy-Szabó,
- Antonieta Cote-Vélez,
- Zoltán Péterfi,
- Magdalini Matziari,
- Mónika Tóth,
- Dóra Zelena,
- Zsolt Mezriczky,
- Andrea Kádár,
- Dóra Kővári,
- Masahiko Watanabe,
- Masanobu Kano,
- Ken Mackie,
- Balázs Rózsa,
- Yvette Ruska,
- Blanka Tóth,
- Zoltán Máté,
- Ferenc Erdélyi,
- Gábor Szabó,
- Balázs Gereben,
- Ronald M. Lechan,
- Jean-Louis Charli,
- Patricia Joseph-Bravo,
- Csaba Fekete
Affiliations
- Erzsébet Farkas
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Edina Varga
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Balázs Kovács
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Anett Szilvásy-Szabó
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Antonieta Cote-Vélez
- Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca 62210, México
- Zoltán Péterfi
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Magdalini Matziari
- Department of Chemistry, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China
- Mónika Tóth
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Dóra Zelena
- Department of Behavioral Neurobiology, Institute of Experimental Medicine, Budapest, Hungary; Centre for Neuroscience, Szentágothai Research Centre, Institute of Physiology, Medical School, University of Pécs, Pécs 7624, Hungary
- Zsolt Mezriczky
- Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest 1088, Hungary
- Andrea Kádár
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Dóra Kővári
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Masahiko Watanabe
- Department of Anatomy, Hokkaido University School of Medicine, Sapporo 060-8638, Japan
- Masanobu Kano
- Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
- Ken Mackie
- Gill Center for Biomolecular Science, Department of Psychological and Brain Sciences, Indiana University, Bloomington 474052, IN, USA
- Balázs Rózsa
- Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Budapest 1083, Hungary
- Yvette Ruska
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Blanka Tóth
- Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellert ter 4, Budapest 1111, Hungary
- Zoltán Máté
- Medical Gene Technology Unit, Institute of Experimental Medicine, Budapest 1083, Hungary
- Ferenc Erdélyi
- Medical Gene Technology Unit, Institute of Experimental Medicine, Budapest 1083, Hungary
- Gábor Szabó
- Medical Gene Technology Unit, Institute of Experimental Medicine, Budapest 1083, Hungary
- Balázs Gereben
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary
- Ronald M. Lechan
- Department of Medicine, Division of Endocrinology, Diabetes and Metabolism, Tupper Research Institute, Tufts Medical Center, Boston 02111, MA, USA; Department of Neuroscience, Tufts University School of Medicine, Boston 02111, MA, USA
- Jean-Louis Charli
- Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca 62210, México
- Patricia Joseph-Bravo
- Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Cuernavaca 62210, México
- Csaba Fekete
- Department of Endocrine Neurobiology, Institute of Experimental Medicine, Szigony u. 43, Budapest 1083, Hungary; Department of Neuroscience, Tufts University School of Medicine, Boston 02111, MA, USA; Corresponding author
- Journal volume & issue
-
Vol. 23,
no. 3
Abstract
Summary: Based on the type-I cannabinoid receptor (CB1) content of hypophysiotropic axons and the involvement of tanycytes in the regulation of the hypothalamic-pituitary-thyroid (HPT) axis, we hypothesized that endocannabinoids are involved in the tanycyte-induced regulation of TRH release in the median eminence (ME). We demonstrated that CB1-immunoreactive TRH axons were associated to DAGLα-immunoreactive tanycyte processes in the external zone of ME and showed that endocannabinoids tonically inhibit the TRH release in this tissue. We showed that glutamate depolarizes the tanycytes, increases their intracellular Ca2+ level and the 2-AG level of the ME via AMPA and kainite receptors and glutamate transport. Using optogenetics, we demonstrated that glutamate released from TRH neurons influences the tanycytes in the ME.In summary, tanycytes regulate TRH secretion in the ME via endocannabinoid release, whereas TRH axons regulate tanycytes by glutamate, suggesting the existence of a reciprocal microcircuit between tanycytes and TRH terminals that controls TRH release. : Molecular Physiology; Neuroscience; Neuroanatomy Subject Areas: Molecular Physiology, Neuroscience, Neuroanatomy
