Nature Communications (Feb 2019)
Improvement of antibody functionality by structure-guided paratope engraftment
- Qingbo Liu,
- Yen-Ting Lai,
- Peng Zhang,
- Mark K. Louder,
- Amarendra Pegu,
- Reda Rawi,
- Mangaiarkarasi Asokan,
- Xuejun Chen,
- Chen-Hsiang Shen,
- Gwo-Yu Chuang,
- Eun Sung Yang,
- Huiyi Miao,
- Yuge Wang,
- Anthony S. Fauci,
- Peter D. Kwong,
- John R. Mascola,
- Paolo Lusso
Affiliations
- Qingbo Liu
- Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, NIH
- Yen-Ting Lai
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Peng Zhang
- Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, NIH
- Mark K. Louder
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Amarendra Pegu
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Reda Rawi
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Mangaiarkarasi Asokan
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Xuejun Chen
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Chen-Hsiang Shen
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Gwo-Yu Chuang
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Eun Sung Yang
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Huiyi Miao
- Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, NIH
- Yuge Wang
- Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, NIH
- Anthony S. Fauci
- Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, NIH
- Peter D. Kwong
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- John R. Mascola
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, NIH
- Paolo Lusso
- Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, NIH
- DOI
- https://doi.org/10.1038/s41467-019-08658-4
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 13
Abstract
Quaternary contacts mediated by an extended heavy-chain framework region 3 (FR3) have been shown to improve binding to HIV envelope and virus neutralization for a few antibodies. Here, Liu et al. engraft such an FR3 loop onto several potent broadly neutralizing antibodies, resulting in improved neutralization activity and pharmacokinetics.
