Metabolomics Analysis Coupled With UPLC/MS on Therapeutic Effect of Jigucao Capsule Against Dampness-Heat Jaundice Syndrome

Yanmei He; Yanmei He; Mengli Zhang; Taiping Li; Taiping Li; Zhien Tan; Aihua Zhang; Min Ou; Danna Huang; Fangfang Wu; Xijun Wang; Xijun Wang

doi:10.3389/fphar.2022.822193

Frontiers in Pharmacology (Jan 2022)

Metabolomics Analysis Coupled With UPLC/MS on Therapeutic Effect of Jigucao Capsule Against Dampness-Heat Jaundice Syndrome

Yanmei He,
Yanmei He,
Mengli Zhang,
Taiping Li,
Taiping Li,
Zhien Tan,
Aihua Zhang,
Min Ou,
Danna Huang,
Fangfang Wu,
Xijun Wang,
Xijun Wang

Affiliations

Yanmei He: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Yanmei He: National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China
Mengli Zhang: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Taiping Li: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Taiping Li: National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China
Zhien Tan: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Aihua Zhang: National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China
Min Ou: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Danna Huang: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Fangfang Wu: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Xijun Wang: National Engineering Laboratory for the Development of Southwestern EndangeredMedicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, China
Xijun Wang: National Chinmedomics Research Center, National TCM Key Laboratory of Serum Pharmacochemistry, Chinmedomics Research Center of State Administration of TCM, Laboratory of Metabolomics, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Harbin, China

DOI: https://doi.org/10.3389/fphar.2022.822193
Journal volume & issue: Vol. 13

Abstract

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Dampness-heat Jaundice Syndrome (DHJS) is a complex Chinese medicine syndrome, while Jigucao capsule (JGCC) is an effective compound preparation of Chinese medicine for the treatment of DHJS about liver and gallbladder, but its mechanism is not clear yet. The purpose of this study is to clarify the pathogenesis of DHJS and the treatment mechanism of JGCC. We used ultra-high performance liquid chromatography/mass spectrometry (UPLC/MS) combined with pattern recognition, accompanied the advanced software and online database for the urine metabolomics of rats. The potential biomarkers disturbing metabolism were identified and the metabolic pathway was analyzed. We investigated the callback of biomarkers after treatment with JGCC. Finally, A total of 25 potential urine biomarkers were identified, including Arachidonic acid, Phenylpyruvic acid, L-Urobilin and so on, and 14 related metabolic pathways were disturbed. After treatment with JGCC, the clinical biochemical indexes and histopathological were significantly improved, and the disturbed biomarkers were also obviously adjusted. It is proved that JGCC has remarkable effect on the treatment of DHJS.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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