Rapid Induction of Astaxanthin in <i>Haematococcus lacustris</i> by Mild Electric Stimulation

Abstract

Efficient induction of astaxanthin (AXT) biosynthesis remains a considerable challenge for the industrialization of the biorefinement of the microalga Haematococcus lacustris. In this study, we evaluated the technical feasibility of photosynthetic electrotreatment to enhance AXT accumulation in H. lacustris. The AXT content of H. lacustris electrotreated at an optimal current intensity (10 mA for 4 h) was 21.8% to 34.9% higher than that of the untreated control group, depending on the physiological state of the initial palmella cells. The contents of other carotenoids (i.e., canthaxanthin, zeaxanthin, and β-carotene) were also increased by this electrotreatment. However, when H. lacustris cells were exposed to more intense electric treatments, particularly at 20 and 30 mA, cell viability significantly decreased to 84.2% and 65.6%, respectively, with a concurrent reduction in the contents of both AXT and the three other carotenoids compared to those of the control group. The cumulative effect of electric stimulation is likely related to two opposing functions of reactive oxygen species, which facilitate AXT biosynthesis as signaling molecules while also causing cellular damage as oxidizing radicals. Collectively, our findings indicate that when adequately controlled, electric stimulation can be an effective and eco-friendly strategy for inducing targeted carotenoid pigments in photosynthetic microalgae.

Keywords

• <i>Haematococcus pluvialis</i>
• <i>Haematococcus lacustris</i>
• astaxanthin
• electric treatment
• oxidative stress
• reactive oxygen species