Immunohistochemical characteristics of MMP-9 and TIMP-1 markers expression in the cerebral cortex in diabetes mellitus type 2

Abstract

Aim – to study the features of immunohistochemical expression of MMP-9, TIMP-1, MMP/TIMP ratio in the cerebral cortex in diabetes mellitus type 2. Materials and methods. A pathomorphological and immunohistochemical study of the section material of brain tissue conducted in 3 groups of observations: group I – control without diabetes and cerebrovascular pathology, group II – deceased patients with dyscirculatory-ischemic encephalopathy (DIE), group III – deceased patients with diabetes mellitus type 2 (DM). Results. Increased expression of MMP-9 in DIE group and in deceased patients with DM type 2 (MMP-9 in the control group – 0.434 (0.399; 0.792) %, in DIE group – 0.652 (0.398; 1.103) %, in DM type 2 group – 0.829 (0.636; 1.034) % has been found. A significant decrease in TIMP-1 expression was detected in DM type 2 (TIMP-1 – in the control group 0.449 (0.254; 0.601) %, in group of DIE – 0.444 (0.306; 0.688) %, in DM type 2 group – 0.278 (0.196; 0.554) %. A statistically significant increase in the MMP/TIMP ratio (Р ˂ 0.05) was observed in DM, the median was 2.732 (1.665; 4.643); this indicator was 1.386 (0.618; 2.29) in the control group and 1.528 (0.619; 3.056) (Р ˃ 0.05) in DIE group. Conclusions. An increase in the level of MMP-9 expression and a decrease in the expression level of TIMP-1 in the cerebral cortex in diabetes mellitus type 2 promote increased blood-brain barrier permeability and is an important link in the pathogenesis of diabetic microangiopathy.

Keywords

• diabetes mellitus
• angiogenesis
• mmp-9
• timp-1
• mmp-9/timp-1