Effects of flavonoids from Allium mongolicum Regel on cell apoptosis, cell cycle and STAT1 mRNA expression in peripheral blood lymphocytes of sheep

Abstract

Flavonoids from Allium mongolicum Regel (AMR) have immunoregulatory and anti-inflammatory properties. However, the effect of flavonoids from A. mongolicum Regel on sheep peripheral blood lymphocytes is still unknown. In this study, we found that total flavonoids from A. mongolicum Regel reduced the apoptosis of peripheral blood lymphocytes in sheep, increased the cell cycle progression to the G2/M phase, and promoted mitosis. These molecules inhibited mRNA expression of the apoptosis-related gene STAT1, indicating that the flavonoids alter JAK-STAT signalling and through this classical pathway inhibit cell apoptosis. The bioactive effects are predominantly related to the structure of flavonoids from A. mongolicum Regel, and the 55% elution group, which contained rutin, quercetin, and mignonette element -5-0-glucose-4-hydroxybenzoic acid, compounds found in plants, was the most effective. The effects were the strongest at a flavonoid concentration of 100μg/ml. In conclusion, the present study showed that flavonoids from A. mongolicum Regel inhibited the expression of STAT1 mRNA, reduced the apoptosis of sheep peripheral blood lymphocytes and promoted cell cycle progression. They also increased the proportion of cells in the G2/M phase. Our findings confirmed a novel role of flavonoids from A. mongolicum Regel in regulating immune function.

Keywords

• flavonoids from allium mongolicum regel
• cell apoptosis
• cell cycle
• stat1
• meat sheep