Serotyping and Cross-Reactivity’s Between Different Pseudomonas aeruginosa Isolates Prevalent in Iran

Abstract

Background and Objectives: 300 Pseudomonas aeruginosa strains were isolated from hospitalized patients in Iran. Using international antigenic typing system (IATS) antibodies, all strains were classified into 16 serotypes while serotype 14 was not identified among the 17 known serotypes. To evaluate the rate of cross-reactivity between O- antigenic determinants, monospecific polyclonal antibodies were made against whole-killed-cells and live cells of each serotype. Material and Methods: Each antiserum was challenged against homologous and heterologous antigens using slide agglutination test. The degree of agglutination reaction is shown by -ve, 1+ve, 2+ve, 3+ve and 4ve for 0, 25%, 50%, 75% and 100% agglutination respectively. Then, the results were tabulated for further study. Results: The rate of cross-reactivity between O-antigenic determinants demonstrated that strains 10.55 and 15.14 had the highest agglutination reaction with serum of all the homologous and heterologous serotypes. Conclusion: Evaluation of the results obtained from the present study can be applied in production of reliable vaccines and antisera as therapeutic agents or as diagnostic kits.

Keywords

• Pseudomonas aeruginosa
• agglutination test
• cross-reactivity
• antigenic schema