Technology for the Bacillus megaterium Fodder Biomass Production

Abstract

Introduction. Obtaining protein and vitamin fodder is one of the urgent tasks that modern industrial biotechnology has to solve. Another task is a search of novel medium compositions for microbial fermentation that can lower production costs. Russian food industry produces a significant amount of sunflower seed processing byproducts every year. Sunflower meal is a promising source of sunflower protein isolate. The research objective was to develop a new technology for the production of Bacillus megaterium bacterial biomass for fodder purposes. Study objects and methods. The research featured a sunflower protein isolate, an enzyme complex Protex 7L, and a B. megaterium strain (VKPM B-3750). The carbohydrate content was determined using a modified Bertrand method. Amine nitrogen was studied using formol titration, the number of viable cells – by the Koch method, the content of amino acids – by capillary electrophoresis. Results and discussion. When processed with enzyme complexes, sunflower protein can be an alternative source of nitrogen for industrial fermentation. The study featured amino acid of sunflower protein isolate and enzymatic hydrolyzate obtained using Protex 7L. A comparative analysis of the content of amino acids in the hydrolyzate and the protein isolate showed that enzymatic hydrolysis can significantly increase the content of free amino acids in the medium available for microbial accumulation. The research proved that sunflower protein enzymatic hydrolyzate obtained using Protex 7L can be used to cultivate strains of B vitamins producers. Conclusion. Sunflower protein enzymatic hydrolyzate can be used as a nitrogen source for B vitamins producer fermentation and as an alternative to expensive meat peptone. The research involved technical and economic assessment of the B. megaterium fermentation on enzymatic hydrolysates of sunflower protein at a production capacity of 100 kg per year. The cost of the protein-vitamin supplement was calculated as 413 rubles per kg, while the market price could reach 826 rubles per kg. The payback period for capital expenditures was estimated at 1.5 years. Thus, replacing commercial meat peptone with sunflower protein enzymatic hydrolyzate obtained with Protex 7L reduced the cost of 1 kg of feed additive by three times without affecting B. megaterium. Overproduction of B vitamins by the B. megaterium strain on a medium containing sunflower protein hydrolyzate requires optimization of fermentation conditions.

Keywords

• sunflower
• sunflower meal
• protein isolate
• fermentation
• enzymatic hydrolysis
• feed additive