Frontiers in Immunology (Aug 2022)

Distinct plasma chemokines and cytokines signatures in Leishmania guyanensis-infected patients with cutaneous leishmaniasis

  • Tirza Gabrielle Ramos de Mesquita,
  • Tirza Gabrielle Ramos de Mesquita,
  • José do Espírito Santo Junior,
  • José do Espírito Santo Junior,
  • Luan Diego Oliveira da Silva,
  • George Allan Villarouco Silva,
  • Felipe Jules de Araújo,
  • Felipe Jules de Araújo,
  • Suzana Kanawati Pinheiro,
  • Suzana Kanawati Pinheiro,
  • Herllon Karllos Athaydes Kerr,
  • Lener Santos da Silva,
  • Lener Santos da Silva,
  • Luciane Macedo de Souza,
  • Samir Assad de Almeida,
  • Krys Layane Guimarães Duarte Queiroz,
  • Josué Lacerda de Souza,
  • Josué Lacerda de Souza,
  • Cilana Chagas da Silva,
  • Héctor David Graterol Sequera,
  • Héctor David Graterol Sequera,
  • Mara Lúcia Gomes de Souza,
  • Mara Lúcia Gomes de Souza,
  • Anderson Nogueira Barbosa,
  • Gemilson Soares Pontes,
  • Gemilson Soares Pontes,
  • Gemilson Soares Pontes,
  • Marcus Vinitius de Farias Guerra,
  • Marcus Vinitius de Farias Guerra,
  • Rajendranath Ramasawmy,
  • Rajendranath Ramasawmy,
  • Rajendranath Ramasawmy,
  • Rajendranath Ramasawmy

DOI
https://doi.org/10.3389/fimmu.2022.974051
Journal volume & issue
Vol. 13

Abstract

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The immunopathology associated with Leishmaniasis is a consequence of inflammation. Upon infection with Leishmania, the type of host-immune response is determinant for the clinical manifestations that can lead to either self-healing or chronic disease. Multiple pathways may determine disease severity. A comparison of systemic immune profiles in patients with cutaneous leishmaniasis caused by L. guyanensis and healthy individuals with the same socio-epidemiological characteristics coming from the same endemic areas as the patients is performed to identify particular immune profile and pathways associated with the progression of disease development. Twenty-seven plasma soluble circulating factors were evaluated between the groups by univariate and multivariate analysis. The following biomarkers pairs IL-17/IL-9 (ρ=0,829), IL-17/IL-12 (ρ=0,786), IL-6/IL-1ra (ρ=0,785), IL-6/IL-12 (ρ=0,780), IL-1β/G-CSF (ρ=0,758) and IL-17/MIP-1β (ρ=0,754) showed the highest correlation mean among the patient while only INF-γ/IL-4 (ρ=0.740), 17/MIP-1β (ρ=0,712) and IL-17/IL-9 (ρ=0,707) exhibited positive correlation among the control group. The cytokine IL-17 and IL1β presented the greater number of positive pair correlation among the patients. The linear combinations of biomarkers displayed IP-10, IL-2 and RANTES as the variables with the higher discriminatory activity in the patient group compared to PDGF, IL-1ra and eotaxin among the control subjects. IP-10, IL-2, IL-1β, RANTES and IL-17 seem to be predictive value of progression to the development of disease among the Lg-infected individuals.

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