Online photochemical derivatization enables comprehensive mass spectrometric analysis of unsaturated phospholipid isomers

Abstract

Mass spectrometry is widely used for large-scale lipid profiling but distinguishing unsaturated lipid isomers is still challenging. Here, the authors present an analytical platform for high-throughput identification of lipid C=C location isomers in clinical samples, showing that isomer ratios may guide biomarker discovery.