Communications Biology (Oct 2021)

Design of functionalised circular tandem repeat proteins with longer repeat topologies and enhanced subunit contact surfaces

  • Jazmine P. Hallinan,
  • Lindsey A. Doyle,
  • Betty W. Shen,
  • Mesfin M. Gewe,
  • Brittany Takushi,
  • Madison A. Kennedy,
  • Della Friend,
  • James M. Roberts,
  • Philip Bradley,
  • Barry L. Stoddard

DOI
https://doi.org/10.1038/s42003-021-02766-y
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 14

Abstract

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Jazmine Hallinan et al. report the development of a new generation of circular tandem repeat proteins with enhanced self-assembly. Functionalisation of these constructs with SARS CoV-2 VHH domains resulted in sub-nanomolar binding affinity to the viral receptor binding domain.