Trophectoderm cell failure leads to peri-implantation lethality in Trpm7-deficient mouse embryos

Aline Schütz; Christin Richter; Petra Weissgerber; Volodymyr Tsvilovskyy; Michael Hesse; Roger Ottenheijm; Frank Zimmermann; Stefanie Buchholz; Rebekka Medert; Sascha Dlugosz; Vladimir Kuryshev; Vladimir Benes; Veit Flockerzi; Bernd K. Fleischmann; Adolfo Cavalié; Marc Freichel

Cell Reports (Oct 2021)

Trophectoderm cell failure leads to peri-implantation lethality in Trpm7-deficient mouse embryos

Aline Schütz,
Christin Richter,
Petra Weissgerber,
Volodymyr Tsvilovskyy,
Michael Hesse,
Roger Ottenheijm,
Frank Zimmermann,
Stefanie Buchholz,
Rebekka Medert,
Sascha Dlugosz,
Vladimir Kuryshev,
Vladimir Benes,
Veit Flockerzi,
Bernd K. Fleischmann,
Adolfo Cavalié,
Marc Freichel

Affiliations

Aline Schütz: Institute of Pharmacology, Heidelberg University, Heidelberg, Germany
Christin Richter: Institute of Pharmacology, Heidelberg University, Heidelberg, Germany
Petra Weissgerber: Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, 66421 Homburg, Germany
Volodymyr Tsvilovskyy: Institute of Pharmacology, Heidelberg University, Heidelberg, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg 69120, Germany
Michael Hesse: Institute of Physiology I, Life and Brain Center, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany
Roger Ottenheijm: Institute of Pharmacology, Heidelberg University, Heidelberg, Germany
Frank Zimmermann: Interfacultary Biomedical Faculty (IBF), University of Heidelberg, Im Neuenheimer Feld 347, 69120 Heidelberg, Germany
Stefanie Buchholz: Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, 66421 Homburg, Germany
Rebekka Medert: Institute of Pharmacology, Heidelberg University, Heidelberg, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg 69120, Germany
Sascha Dlugosz: Interfacultary Biomedical Faculty (IBF), University of Heidelberg, Im Neuenheimer Feld 347, 69120 Heidelberg, Germany
Vladimir Kuryshev: Institute of Pharmacology, Heidelberg University, Heidelberg, Germany
Vladimir Benes: Developmental Biology Unit, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Veit Flockerzi: Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, 66421 Homburg, Germany
Bernd K. Fleischmann: Institute of Physiology I, Life and Brain Center, Medical Faculty, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany
Adolfo Cavalié: Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, 66421 Homburg, Germany
Marc Freichel: Institute of Pharmacology, Heidelberg University, Heidelberg, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg 69120, Germany; Corresponding author

Journal volume & issue: Vol. 37, no. 3
p. 109851

Abstract

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Summary: Early embryogenesis depends on proper control of intracellular homeostasis of ions including Ca2+ and Mg2+. Deletion of the Ca2+ and Mg2+ conducting the TRPM7 channel is embryonically lethal in mice but leaves compaction, blastomere polarization, blastocoel formation, and correct specification of the lineages of the trophectoderm and inner cell mass unaltered despite that free cytoplasmic Ca2+ and Mg2+ is reduced at the two-cell stage. Although Trpm7−/− embryos are able to hatch from the zona pellucida, no expansion of Trpm7−/− trophoblast cells can be observed, and Trpm7−/− embryos are not identifiable in utero at E6.5 or later. Given the proliferation and adhesion defect of Trpm7−/− trophoblast stem cells and the ability of Trpm7−/− ESCs to develop to embryos in tetraploid embryo complementation assays, we postulate a critical role of TRPM7 in trophectoderm cells and their failure during implantation as the most likely explanation of the developmental arrest of Trpm7-deficient mouse embryos.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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