Frontiers in Plant Science (Jul 2017)

Mapping QTL for Seed Germinability under Low Temperature Using a New High-Density Genetic Map of Rice

  • Ningfei Jiang,
  • Ningfei Jiang,
  • Shilai Shi,
  • Shilai Shi,
  • Huan Shi,
  • Huan Shi,
  • Hira Khanzada,
  • Ghulam M. Wassan,
  • Changlan Zhu,
  • Changlan Zhu,
  • Changlan Zhu,
  • Xiaosong Peng,
  • Xiaosong Peng,
  • Qiuying Yu,
  • Xiaorong Chen,
  • Xiaorong Chen,
  • Xiaopeng He,
  • Xiaopeng He,
  • Junru Fu,
  • Junru Fu,
  • Lifang Hu,
  • Lifang Hu,
  • Lifang Hu,
  • Jie Xu,
  • Jie Xu,
  • Linjuan Ouyang,
  • Linjuan Ouyang,
  • Xiaotang Sun,
  • Xiaotang Sun,
  • Xiaotang Sun,
  • Dahu Zhou,
  • Dahu Zhou,
  • Haohua He,
  • Haohua He,
  • Haohua He,
  • Jianmin Bian,
  • Jianmin Bian,
  • Jianmin Bian

DOI
https://doi.org/10.3389/fpls.2017.01223
Journal volume & issue
Vol. 8

Abstract

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Mapping major quantitative trait loci (QTL) responsible for rice seed germinability under low temperature (GULT) can provide valuable genetic source for improving cold tolerance in rice breeding. In this study, 124 rice backcross recombinant inbred lines (BRILs) derived from a cross indica cv. Changhui 891 and japonica cv. 02428 were genotyped through re-sequencing technology. A bin map was generated which includes 3057 bins covering distance of 1266.5 cM with an average of 0.41 cM between markers. On the basis of newly constructed high-density genetic map, six QTL were detected ranging from 40 to 140 kb on Nipponbare genome. Among these, two QTL qCGR8 and qGRR11 alleles shared by 02428 could increase GULT and seed germination recovery rate after cold stress, respectively. However, qNGR1 and qNGR4 may be two major QTL affecting indica Changhui 891germination under normal condition. QTL qGRR1 and qGRR8 affected the seed germination recovery rate after cold stress and the alleles with increasing effects were shared by the Changhui 891 could improve seed germination rate after cold stress dramatically. These QTL could be a highly valuable genetic factors for cold tolerance improvement in rice lines. Moreover, the BRILs developed in this study will serve as an appropriate choice for mapping and studying genetic basis of rice complex traits.

Keywords