Generation of a human induced pluripotent stem cell line, KSCBi003-A, from human adipose tissue-derived mesenchymal stem cells using a chromosomal integration-free system

Hye Young Choi; So-Jung Kim; Gue Youn Go; Ara Kwon; Young Sam Im; Hye-Yeong Ha; Jin Tae Hong; Ji-won Jung; Soo Kyung Koo

Stem Cell Research (Aug 2018)

Generation of a human induced pluripotent stem cell line, KSCBi003-A, from human adipose tissue-derived mesenchymal stem cells using a chromosomal integration-free system

Hye Young Choi,
So-Jung Kim,
Gue Youn Go,
Ara Kwon,
Young Sam Im,
Hye-Yeong Ha,
Jin Tae Hong,
Ji-won Jung,
Soo Kyung Koo

Affiliations

Hye Young Choi: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea; College of Pharmacy, Chungbuk National University, Chungcheongbuk-do, South Korea
So-Jung Kim: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea
Gue Youn Go: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea
Ara Kwon: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea
Young Sam Im: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea
Hye-Yeong Ha: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea
Jin Tae Hong: College of Pharmacy, Chungbuk National University, Chungcheongbuk-do, South Korea
Ji-won Jung: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea
Soo Kyung Koo: Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea; Corresponding author.

Journal volume & issue: Vol. 31
pp. 1 – 4

Abstract

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We generated a human induced pluripotent stem cell (hiPSC) line, KSCBi003-A, from adipose tissue-derived mesenchymal stem cells (Ad-MSCs) using a Sendai virus-based gene delivery system. We confirmed that the KSCBi003-A has a normal karyotype and short tandem repeat (STR)-based identities that match the parent cells. We also confirmed that the cell line expresses pluripotent stem cell markers such as Nanog, OCT4, SSEA-4, TRA-1-60, and TRA-1-81. We also analyzed that the KSCBi003-A has an ability to differentiate three germ layers (ectoderm, mesoderm, endoderm). This cell line is registered and available at the National Stem Cell Bank, Korea National Institute of Health.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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