Mg-Fe Layered Double Hydroxides Enhance Surfactin Production in Bacterial Cells

Abstract

In this study, four additives—montmorillonite, activated carbon, and the layered double hydroxides (LDHs), Mg2Fe−LDH and Mg2Al−LDH—were tested for their ability to promote surfactin production in a Bacillus subtilis ATCC 21332 culture. Among these tested materials, the addition of 4 g/L of the Mg-Fe LDH, which featured an Mg/Fe molar ratio of 2:1, produced the highest surfactin yield of 5280 mg/L. During the time course of B. subtilis cultivation with the added LDH, two phases of cell growth were evident: Growth and decay. In the growth phase, the cells grew slowly and secreted a high amount of surfactin; in the decay phase, the cells degraded rapidly. The production in the presence of the Mg2Fe−LDH had three characteristics: (i) High surfactin production at low biomass, indicating a high specific surfactin yield of 3.19 g/g DCW; (ii) rapid surfactin production within 24 h, inferring remarkably high productivity (4660 mg/L/d); and (iii) a lower carbon source flux to biomass, suggesting an efficient carbon flux to surfactin, giving a high carbon yield of 52.8%. The addition of Mg2Fe−LDH is an effective means of enhancing surfactin production, with many potential applications and future industrial scale-up.

Keywords

• <i>Bacillus subtilis</i>
• surfactin
• quantitative analysis
• fermentation
• growth phase
• layered double hydroxides