Revista Brasileira de Ortopedia (Feb 2016)

Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes

  • Lívia Maria Mendonça Augusto,
  • Diego Pinheiro Aguiar,
  • Danielle Cabral Bonfim,
  • Amanda dos Santos Cavalcanti,
  • Priscila Ladeira Casado,
  • Maria Eugênia Leite Duarte

DOI
https://doi.org/10.1016/j.rboe.2015.12.013
Journal volume & issue
Vol. 51, no. 1
pp. 70 – 74

Abstract

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ABSTRACT OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.

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