Molecular design strategy to alleviate environmental interference on two-photon fluorescence probes

Xingxing Zhang; Tianbing Ren; Qianling Zhang; Ting Zeng; Zhe Li; Feiyu Yang; Xiaobing Zhang; Lin Yuan

Cell Reports Physical Science (Jun 2021)

Molecular design strategy to alleviate environmental interference on two-photon fluorescence probes

Xingxing Zhang,
Tianbing Ren,
Qianling Zhang,
Ting Zeng,
Zhe Li,
Feiyu Yang,
Xiaobing Zhang,
Lin Yuan

Affiliations

Xingxing Zhang: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Tianbing Ren: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Qianling Zhang: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Ting Zeng: College of Life Science, Hunan Normal University, Changsha 410082, China
Zhe Li: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Feiyu Yang: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Xiaobing Zhang: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Lin Yuan: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China; Corresponding author

Journal volume & issue: Vol. 2, no. 6
p. 100471

Abstract

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Summary: Electron donor-acceptor (D-A)-type molecules are the most widely employed two-photon scaffolds. Unfortunately, current D-A-type fluorophores suffer from serious solvent-dependent emission wavelength and brightness, thwarting their use for high-fidelity imaging in complicated biological systems. Herein, with single-atom replacement and acceptor fusing in acedan (6-acetyl-2-(dimethylamino)naphthalene), we devise a new class of D-A type fluorophore (TPQL dyes). Our design results in TPQL fluorophores with a slight emission redshift (ΔλTPQL1 < 32 nm versus ΔλAcedan-NH2 = 91 nm) and high brightness (εΦTPQL1 = 7,600 to 6,923 versus εΦAcedan-NH2 = 7,372 to 1,314) in different solvent systems. Such features not only provide bright and unbiased fluorescent images of TPQL fluorophores in the cell but also bring deeper tissue penetration and greater signal-to-background ratios in tissue. As a proof-of-concept demonstration, using probe TPQL-N3 and TPQL-APN, we monitor the dynamic changes of H2S and APN during zebrafish liver development with high fidelity to the best of our knowledge.

Published in Cell Reports Physical Science

ISSN: 2666-3864 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Physics
Website: https://www.cell.com/cell-reports-physical-science

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