Chromatin complex dependencies reveal targeting opportunities in leukemia

Fadi J. Najm; Peter DeWeirdt; Molly M. Moore; Samantha M. Bevill; Chadi A. El Farran; Kevin A. Macias; Mudra Hegde; Amanda L. Waterbury; Brian B. Liau; Peter van Galen; John G. Doench; Bradley E. Bernstein

doi:10.1038/s41467-023-36150-7

Nature Communications (Jan 2023)

Chromatin complex dependencies reveal targeting opportunities in leukemia

Fadi J. Najm,
Peter DeWeirdt,
Molly M. Moore,
Samantha M. Bevill,
Chadi A. El Farran,
Kevin A. Macias,
Mudra Hegde,
Amanda L. Waterbury,
Brian B. Liau,
Peter van Galen,
John G. Doench,
Bradley E. Bernstein

Affiliations

Fadi J. Najm: Gene Regulation Observatory, Broad Institute of MIT and Harvard
Peter DeWeirdt: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Molly M. Moore: Gene Regulation Observatory, Broad Institute of MIT and Harvard
Samantha M. Bevill: Gene Regulation Observatory, Broad Institute of MIT and Harvard
Chadi A. El Farran: Gene Regulation Observatory, Broad Institute of MIT and Harvard
Kevin A. Macias: Gene Regulation Observatory, Broad Institute of MIT and Harvard
Mudra Hegde: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Amanda L. Waterbury: Gene Regulation Observatory, Broad Institute of MIT and Harvard
Brian B. Liau: Gene Regulation Observatory, Broad Institute of MIT and Harvard
Peter van Galen: Division of Hematology, Brigham and Women’s Hospital
John G. Doench: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Bradley E. Bernstein: Gene Regulation Observatory, Broad Institute of MIT and Harvard

DOI: https://doi.org/10.1038/s41467-023-36150-7
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 10

Abstract

Read online

Epigenetic regulators are potential therapeutic drug targets in leukemia. Here, the authors perform combinatorial CRISPR knockouts to test gene-gene pairings in leukemia cells to discover compensatory non-lethal or synergistic lethal combinations with therapeutic potential.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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