Microbiologia Medica (Mar 2010)

Evaluation of a new system (RAD 120) for the serologic determination of the T.O.R.C.H complex analytes

  • Mauro Carcheri,
  • Ilaria Chiossone,
  • Paola Milano,
  • Anna Alabiso,
  • Fabrizia Capanna,
  • Annamaria Ferretti,
  • Licia Muselli,
  • Caterina Oliveri,
  • Laura Tassi,
  • Roberto Capuzzo

DOI
https://doi.org/10.4081/mm.2010.2477
Journal volume & issue
Vol. 25, no. 1

Abstract

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The aim of this study was to evaluate the results of the determination of IgG and IgM against TORCH complex antigens obtained by using a new automatic random-access (RAD 120 – RADIM/SEAC) instrument based on FEIA method, in comparison with the results obtained for the same analytes by using a traditional analyzer based on a coated microplate ELISA method (ALISEI-RADIM/SEAC).The analysis of the discordances between the results was made with the system ELFA (VIDAS – bioMérieux), taken as a reference test. The general concordance of the results obtained with the FEIA and the ELISA was over 97.00%. For the single analyte as Toxo IgG,Toxo IgM, Rubeo IgM, CMV IgG, CMV IgM and HSV 2 IgG, the concordance was above 96.00% and 100.00% for the Rubeo IgG and HSV 1 IgG. For the HSV 1/2 IgM, the FEIA/ELISA results show lower concordance, but still reaching 90.16%. For the IgG and IgM dosage, the FEIA seems to give more positive results than the ELISA. If the discordances FEIA/ELISA concern the IgG, the ELISA seems to agree better with the ELFA results. For the IgM dosage, all the FEIA positive/ELISA negative results have been confirmed as negative at ELFA. In conclusion the new technology FEIA/RAD120 gave results which can be comparable with those obtained at the traditional ELISA as well at the alternative ELFA.

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