ANTIOXIDANT CAPACITY AND TOTAL PHENOLS CONTENT CHANGES ON CRESS (LEPIDIUM SATIVUM) SPROUTS AFTER EXOGENOUS SUPPLY WITH NANO SELENIUM

Abstract

Selenium is an essential trace element for human health due to its different biological activities. Our aim was to investigate the effects of nano-selenium particles (NSePs) supply on cress sprouts from point of view of total phenols content and antioxidant capacity of selenium – enriched cress sprouts. The NSePs were produced by chemical reduction of NaHSeO3 with glucose. Cress seeds were germinated in the plastic boxes, sprinkled every day with different concentrations of NSePs solution (10, 50, 100 ppm) for 8 days. The length of shoots and roots were measured, and the total phenols content was determined by Folin-Ciocalteu method, while antioxidant capacity was evaluated by DPPH assay. By NSePs supply, the growth parameters of cress sprouts were not affected compared with the control sample. Total phenol content of shoots was not affected by treatment with NSePs at 10 and 100 ppm, but 50 ppm NSePs supply increased the total phenols content compared with the control. The antioxidant capacity of shoots was increased significantly (P=0,05) in the samples treated with 10 and 100 ppm NSePs. Using NSePs as fertilizer, selenium enriched cress sprouts can be obtained, with positive effects on human health.

Keywords

• nano-selenium
• antioxiyesnt capacity
• cress