Direct detection of rpoB and katG gene mutations of Mycobacterium tuberculosis in clinical samples

Abstract

Objectives: To study the rpoB and katG gene mutation rate and its markers. Methods: Cross-sectional study methods were used to study Tuberculosis. A total of 45 sputum samples were collected from Annapurna Neurological Institute and Allied sciences. Then, acid fast bacilli staining were performed. Positive and negative samples were carried for conventional polymerase chain reaction identification and electrophoresis. Results: Out of 45 samples, 3 were acid fast bacilli positive and the rest were negative. Male participants were more as compare to female participants and the mutation in rpoB and katG gene was found similar i.e. 6.66% among the total samples. Conclusions: We can conclude that genetic mutation in Mycobacterium tuberculosis can be identified directly from the clinical samples. However, we have carried this study in less sample size and to validate research on large number of sample is recommended.

Keywords

• Mycobacterium tuberculosis
• Direct detection
• rpoB
• katG
• Mutation
• Nepal