OCORRÊNCIA E ATIVIDADE DE BACTÉRIAS DIAZOTRÓFICAS EM FORRAGEIRAS CULTIVADAS NA REGIÃO SEMIÁRIDA NO BRASIL

Abstract

Diazotrophic bacteria play important role on economy nitrogen fertilizer in forage glasses and environmental sustainability. With the objective of evaluate the occurrence of diazotrophic bacteria associated associated with native forage at two different times and identify isolates with potential for fixing N2 in the semiarid region. Was conducted in the Soil Laboratory of the Centro de Saúde e Tecnologia Rural in Federal University of Campina Grande, Campus de Patos, Paraíba. A study was carried out at the Soil Laboratory of the Centro de Saúde e Tecnologia Rural of the Federal University of Campina Grande, in Patos, Paraíba. The objective this study was to verify in the native diazotrophic endophytic bacteria associated with forage glasses and to identify isolates in NBF hability, during the rainy and dry seasons in semiarid region. Root samples of three forage grasses [Andropogon gayanus Kunth (Andropogon), Panicum maximum Jacq. cv. Tanzania (Tanzânia) and Cenchrus ciliaris L. (Buffel)] cultivated in the field experiment was utilized. The bacteria were isolated in NFb culture media free-N specific for Azospirillum spp., the isolates was quantified, phenotypically characterized, purified. From caracterized after, was evaluated for their nitrogenase activity in vitro. The resultas show diazotrophics bacteria growth in the three forage grasses tested. The ocorrency diazotrophics bacteria be influencied by genotipic plant and seasonal variation. A more density of NSDB of the genus Azospirillum associated in the root grasses were obtained in dry season. The NSDB isolates, show a high potencial for new studies on the genetic caracteristcs and avaliation the inoculo potential to (BNF) associated on grasses in semiarid region.