Jasmonic acid and salicylic acid induced defensive response in wine grapes against Drosophila suzukii (Diptera: Drosophilidae)

Abstract

To better understand the elicitor induced defense in wine grapes against Drosophila suzukii (Matsumura), two varieties, Pinot Noir and Chardonnay, were sprayed with jasmonic acid (JA) and salicylic acid (SA). Total phenols, total flavonoids, total tannins and total soluble sugars were quantified. Oviposition preference by D. suzukii in response to JA and SA applications were also studied. Behavioral response of D. suzukii to various sugars was recorded. The effect of flavonoids (gallic acid, catechin, quercetin at 100 and 500 ppm) on mortality of D. suzukii was also studied in CAFE assay. Our results showed that JA and SA application significantly affected phenol, flavonoid and tannin contents of grapes. Reduced injury was observed in treated plants; this reduction was more pronounced in Chardonnay than Pinot Noir. The number of eggs laid by D. suzukii females was lower in JA and SA-treated plants; this reduction was more pronounced under no-choice conditions than under choice conditions. In prandial behavior, sucrose solution (5%), glucose solution (5%), fructose solution (5%), sucrose (5%) + yeast (5%) solutions and yeast solutions (5%) showed higher attraction of D. suzukii females compared to control treatments. Among flavonoids, catechin (100 ppm) showed higher mortality of D. suzukii than rest of the treatments. The results of this study can be used for developing management strategies of D. suzukii in wine grapes and related crops.

Keywords

• Spotted-wing drosophila
• Signaling molecules
• Secondary metabolites
• Wine grape
• Pest management