In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy

Thomas Kupke; Rabea M. Götz; Florian M. Richter; Rainer Beck; Fabio Lolicato; Walter Nickel; Carsten Hopf; Britta Brügger

doi:10.1038/s42003-023-04654-z

Communications Biology (Mar 2023)

In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy

Thomas Kupke,
Rabea M. Götz,
Florian M. Richter,
Rainer Beck,
Fabio Lolicato,
Walter Nickel,
Carsten Hopf,
Britta Brügger

Affiliations

Thomas Kupke: Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328
Rabea M. Götz: Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences
Florian M. Richter: Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences
Rainer Beck: Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328
Fabio Lolicato: Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328
Walter Nickel: Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328
Carsten Hopf: Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences
Britta Brügger: Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328

DOI: https://doi.org/10.1038/s42003-023-04654-z
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 15

Abstract

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iCLiPSpy is a simple assay to identify residues important for substrate binding and activity of intramembrane-cleaving proteases (I-CLiPs) in vivo and can be adapted to a screening assay for I-CLiP inhibitor or activator discovery.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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