مجله دانشکده پزشکی اصفهان (Jan 2017)
Prognostic Importance of Polymorphisms in DNA-Repair Gene (XRCC1 Arg399Gln) in Patients with Lung Cancer in Fars Province, Iran
Abstract
Background: Genetic polymorphisms in DNA-repair genes may increase the risk of developing cancer due to reducing the DNA-repair capacity. XRCC1 is one of the important genes in DNA repair. This study was designed to examine the polymorphisms associated with XRCC1 Arg399Gln and to investigate its role as susceptibility marker for non-small cell lung cancer (NSCLC) in population of Fars province, Iran. Methods: In this case-control study, extracted DNA from 100 healthy controls and 100 patient of lung cancer were used to examine the role of XCRR1 Arg399Gln polymorphism in context of non-small cell lung cancer in population of Fars province in Iran. Amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for determining of individuals’ genotyping. Findings: Our data showed a strong association between Gln/Gln (A/A) genotype and risk of developing non-small cell lung cancer in men. Moreover, men with at least one A allele (AA+ AG) showed reduced risk of developing non-small cell lung cancer. No such an associations were found in subgroups of women or when samples were divided based on their ages. Conclusion: According to our results, although there was no significant association between XRCC1 Age399Gln polymorphism and developing of lung cancer in population, men with Gln/Gln genotype were in high risk of developing non-small cell lung cancer in Fars province, Iran. Therefore, Gln/Gln polymorphism could be used as a biomarker for screening men at high risk of lung cancer.
