Prevalence of canine tick-borne haemoparasites in three Divisional Secretariat Divisions (Rambewa, Tirappane, and Galenbidunuwewa) in the Anuradhapura district, Sri Lanka

Abstract

Introduction and Objectives: Ticks act as the vector for transmission of many parasitic pathogens. Several tick-borne haemoparasite infections are widespread among canines in tropical countries. However, information is scarce regarding canine tick-borne infections in Sri Lanka. The present study was therefore carried out to identify the canine tick-borne haemoparasite species and thereby assess their prevalence in three Divisional Secretariat divisions in the Anuradhapura district.Materials and methods: Blood samples were collected from dogs in three Divisional Secretariat (DS) divisions namely, Rambewa, Tirappane, and Galenbindunuwewa in the Anuradhapura district, Sri Lanka. From each blood sample, two thin smears were prepared and stained with Giemsa. Stained slides were subsequently examined with a light microscope to detect haemoparasites.Results: Out of 319 blood samples, 139 were positive for haemoparasites. Of the positives, 51.61% were from Rambewa, 40.47% were from Galenbindunuwewa and 38.88% were from Tirappane. Three haemoprotozoan species, Hepatozoon canis, Babesia gibsoni, Babesia canis and one bacterial species, Ehrlichia canis were morphologically identified in the present study.Conclusions: Comparatively, the prevalence of ehrlichiosis (20.06%) and babesiosis (16.29%) were high in three DS divisions compared to the prevalence of H. canis infection (1.56%). The prevalence of the haemoprotozoan infections was significantly different among thee DS divisions (P<0.01). Co-infections among dogs suggest that the same vector may be involved in transmitting different infections.

Keywords

• haemoprotozoa, hepatozoon canis, babesia gibsoni, babesia canis, ehrlichia canis