The Protective Role of Syringic Acid on Ovarian Injury Created by Ischemia Reperfusion

Abstract

INTRODUCTION: Syringic acid (SA), a natural phenolic substance, possesses anti-inflammatory and antioxidant properties. We investigated the likely preventive effects of SA on organ damage to the reproductive system caused by bilateral ovarian ischemia-reperfusion (I/R). METHODS: 32 female Sprague-Dawley rats in total were used; they were split into four groups: a sham group for control (group I), I/R group (group II), I/R group given 10 mg/kg SA (group III), and an I/R group given 50 mg/kg SA (group IV). Group I underwent only an abdominal incision without I/R. In groups II, III, and IV, I/R procedures were conducted for 3 hours. SA was given intraperitoneally to groups III and IV for 30 minutes before reperfusion at 10 mg/kg and 50 mg/kg, respectively. The ovarian tissues were surgically removed for analysis. RESULTS: TNF-α, MDA, IL-1β, MPO, TOS and OSI levels in group II were found to be significantly higher than in group I. Additionally, a decrease was detected in TAS and SOD values in group II. In contrast, SA administration led to significant increases in the level of antioxidant enzymes in both groups III and IV. Also, in the SA-given groups, significant decreases were detected in OSI and TOS levels, along with a decrease in MPO, MDA, TNF-α, and IL-1β levels. DISCUSSION AND CONCLUSION: It has been demonstrated that SA has a protective effect against organ damage caused by bilateral ovarian I/R in the reproductive system. This is the first study to show that SA can reduce ovarian damage due to I/R injury.

Keywords

• ischemia reperfusion
• syringic acid
• ovary
• oxidative stress.