Morphological and histochemical changes in the brain stem in case of experimental hemispheric intracerebral hemorrhage

Abstract

Aim. Investigation of the extent of morphological changes and activity of biogenic amines (according to the intensity of luminescence) in the neurons of the brain stem in intracerebral hemorrhage (ICH). Methods and results. ICH was designed on 29 white rats of Vistar line by the administration of autologous blood in the cerebral hemisphere. It was revealed that increased luminescence intensity by 18.4±5.5% was registered in monoaminergic neurons in 1–6 hours after experimental ICH. After 12 hours – 1 day development of dislocation syndrome leads to mosaic focal ischemic neuronal injuries with maximum reduction in the level of catecholamines by 29.5±5.0% compared with control cases. Three–6 days after ICH on a background of selective neuronal necrosis in substantial number of neurons in the nuclei of the brainstem the level of catecholamines is significantly reduced. Conclusion. Disclosed observations reflect significant functional pathology of neurons responsible for the regulation of cardiorespiratory function and may underlie disturbances of integrative activity in the brain stem in general.

Keywords

• Experimentation Animal
• Brainstem
• Monoaminergic Neurons
• Histochemistry
• Cerebral Hemorrhage