Large-scale bulk RNA-seq analysis defines immune evasion mechanism related to mast cell in gliomas

Rui Chen; Wantao Wu; Wantao Wu; Wantao Wu; Tao Liu; Tao Liu; Tao Liu; Yihan Zhao; Yihan Zhao; Yihan Zhao; Yifan Wang; Yifan Wang; Hao Zhang; Hao Zhang; Zeyu Wang; Zeyu Wang; Ziyu Dai; Ziyu Dai; Xiaoxi Zhou; Xiaoxi Zhou; Peng Luo; Jian Zhang; Zaoqu Liu; Li-Yang Zhang; Li-Yang Zhang; Quan Cheng; Quan Cheng

doi:10.3389/fimmu.2022.914001

Frontiers in Immunology (Sep 2022)

Large-scale bulk RNA-seq analysis defines immune evasion mechanism related to mast cell in gliomas

Rui Chen,
Wantao Wu,
Wantao Wu,
Wantao Wu,
Tao Liu,
Tao Liu,
Tao Liu,
Yihan Zhao,
Yihan Zhao,
Yihan Zhao,
Yifan Wang,
Yifan Wang,
Hao Zhang,
Hao Zhang,
Zeyu Wang,
Zeyu Wang,
Ziyu Dai,
Ziyu Dai,
Xiaoxi Zhou,
Xiaoxi Zhou,
Peng Luo,
Jian Zhang,
Zaoqu Liu,
Li-Yang Zhang,
Li-Yang Zhang,
Quan Cheng,
Quan Cheng

Affiliations

Rui Chen: Department of Neurosurgery, Affiliated Nanhua Hospital, University of South China, Hengyang, China
Wantao Wu: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, China
Wantao Wu: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Wantao Wu: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China
Tao Liu: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Tao Liu: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China
Tao Liu: Xiangya School of Medicine, Central South University, Changsha, China
Yihan Zhao: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Yihan Zhao: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China
Yihan Zhao: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, China
Yifan Wang: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Yifan Wang: Xiangya School of Medicine, Central South University, Changsha, China
Hao Zhang: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Hao Zhang: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China
Zeyu Wang: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Zeyu Wang: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China
Ziyu Dai: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Ziyu Dai: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China
Xiaoxi Zhou: Department of Neurosurgery, Affiliated Nanhua Hospital, University of South China, Hengyang, China
Xiaoxi Zhou: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Peng Luo: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, China
Jian Zhang: Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou, China
Zaoqu Liu: Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou, Zhengzhou, China
Li-Yang Zhang: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Li-Yang Zhang: Xiangya School of Medicine, Central South University, Changsha, China
Quan Cheng: National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Quan Cheng: Xiangya School of Medicine, Central South University, Changsha, China

DOI: https://doi.org/10.3389/fimmu.2022.914001
Journal volume & issue: Vol. 13

Abstract

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Accumulating evidence has demonstrated that the immune cells have an emerging role in controlling anti-tumor immune responses and tumor progression. The comprehensive role of mast cell in glioma has not been illustrated yet. In this study, 1,991 diffuse glioma samples were collected from The Cancer Genome Atlas (TCGA) and the Chinese Glioma Genome Atlas (CGGA). xCell algorithm was employed to define the mast cell-related genes. Based on mast cell-related genes, gliomas were divided into two clusters with distinct clinical and immunological characteristics. The survival probability of cluster 1 was significantly lower than that of cluster 2 in the TCGA dataset, three CGGA datasets, and the Xiangya cohort. Meanwhile, the hypoxic and metabolic pathways were active in cluster 1, which were beneficial to the proliferation of tumor cells. A potent prognostic model based on mast cell was constructed. Via machine learning, DRG2 was screened out as a characteristic gene, which was demonstrated to predict treatment response and predict survival outcome in the Xiangya cohort. In conclusion, mast cells could be used as a potential effective prognostic factor for gliomas.

Published in Frontiers in Immunology

ISSN: 1664-3224 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: http://journal.frontiersin.org/journal/immunology

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