Traumatic temporomandibular joint bony ankylosis in growing rats

Zhen Ma; Yiming Wang; Yang Xue; Wuyang Zhang; Dengke Li; Yuan Li; Guowei Li; Hongzhi Zhou; Xiangxiang Hu; Tiange Deng; Kaijin Hu

doi:10.1186/s12903-022-02560-0

BMC Oral Health (Dec 2022)

Traumatic temporomandibular joint bony ankylosis in growing rats

Zhen Ma,
Yiming Wang,
Yang Xue,
Wuyang Zhang,
Dengke Li,
Yuan Li,
Guowei Li,
Hongzhi Zhou,
Xiangxiang Hu,
Tiange Deng,
Kaijin Hu

Affiliations

Zhen Ma: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Yiming Wang: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Yang Xue: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Wuyang Zhang: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Dengke Li: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Yuan Li: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Guowei Li: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Hongzhi Zhou: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Xiangxiang Hu: Division of Oral and Craniofacial Health Sciences, University of North Carolina Adams School of Dentistry
Tiange Deng: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University
Kaijin Hu: State Key Laboratory of Military Stomatology, National Clinical Research and Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases and Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University

DOI: https://doi.org/10.1186/s12903-022-02560-0
Journal volume & issue: Vol. 22, no. 1
pp. 1 – 13

Abstract

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Abstract Background The pathogenesis of traumatic temporomandibular joint (TMJ) bony ankylosis remains unknown. This study aimed to explore the pathogenesis of traumatic TMJ bony ankylosis in a rat model. Methods Twenty-four 3-week-old male Sprague–Dawley rats were used in this study. Excision of the whole disc, the fibrocartilage damage of the condyle and glenoid fossa, and narrowed joint space were performed in the left TMJ of the operation group to induce TMJ bony ankylosis (experimental side). The right TMJ underwent a sham operation (sham side). The control group did not undergo any operations. At 1, 4, and 8 weeks postoperatively, rats of the operation group were sacrificed and TMJ complexes were evaluated by gross observation, Micro-CT, histological examinations, and immunofluorescence microscopy. Total RNA of TMJ complexes in the operation group were analyzed using RNA-seq. Results Gross observations revealed TMJ bony ankylosis on the experimental side. Micro-CT analysis demonstrated that compared to the sham side, the experimental side showed a larger volume of growth, and a considerable calcified bone callus formation in the narrowed joint space and on the rougher articular surfaces. Histological examinations indicated that endochondral ossification was observed on the experimental side, but not on the sham side. RNA-seq analysis and immunofluorescence revealed that Matrix metallopeptidase 13 (MMP13) and Runt-related transcription factor 2 (RUNX2) genes of endochondral ossification were significantly more downregulated on the experimental side than on the sham side. The primary pathways related to endochondral ossification were Parathyroid hormone synthesis, secretion and action, Relaxin signaling pathway, and IL-17 signaling pathway. Conclusions The present study provided an innovative and reliable rat model of TMJ bony ankylosis by compound trauma and narrowed joint space. Furthermore, we demonstrated the downregulation of MMP13 and RUNX2 in the process of endochondral ossification in TMJ bony ankylosis.

Published in BMC Oral Health

ISSN: 1472-6831 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Medicine: Dentistry
Website: http://bmcoralhealth.biomedcentral.com

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